anti atf3 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology anti atf3
Anti Atf3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+atf3/ATF-3+Antibody/bio_rxiv__64898__2026__02__23__707492-338-12-13
Average 94 stars, based on 640 article reviews

anti atf3 - by Bioz Stars, 2026-10

94/100 stars

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Incubation:

Article Title: Single-Nucleus Chromatin Accessibility and Epigenetic Study Uncover Cell States and Transcriptional Regulation of Epidermis in Hidradenitis Suppurativa
Article Snippet: .. The sections were then incubated with primary antibodies against proteins for anti-Krt14 (1:400, Biolegend, San Diego, CA, USA, Cat# 906004) and anti-ATF3 (1:50, Santa Cruz, Dallas, TX, USA, Catsc-518032) in blocking solution overnight at 4 °C. ..

Article Title: Vascular Smooth Muscle Cell-Specific BCAT2 Deficiency Attenuates Diabetic Atherosclerotic Calcification via Histone Propionylation
Article Snippet: .. Samples were incubated with primary antibodies, including anti-BCAT2 ( Ab309514 , Abcam, USA), anti-Kpr (PTM-203, Proteomics, China), anti-H3K23pr (PTM-208, Proteomics, China), anti-ATF3 (sc-518032, Santa Cruz, USA), or anti-α-SMA (BM0002, Boster, China) at 4 °C overnight followed by incubation with corresponding secondary antibody. ..

Article Title: Sciatic nerve stimulation alleviates neuropathic pain and associated neuroinflammation in the dorsal root ganglia in a rodent model.
Article Snippet: Twenty-μm cryosections of the DRG were cut using a cryostat (CM1950; LEICA, Wetzlar, Germany) at −20 °C for further immunofluorescence. .. Sections were permeabilized and blocked with 10% normal goat serum (prepared in PBS supplemented with 0.1% bovine serum albumin and 0.1% Triton X-100) for 20 min. Next, the sections were incubated overnight at 4 °C with the following primary antibodies: anti-GFAP (G3893, 1:500; Sigma-Aldrich), anti-S100A10 (11250- 1-AP, 1:200; Proteintech), anti-NeuN (MAB377, 1:200; Merck), anti-NeuN (MABN140, 1:200; Merck), antiTRPA1 (ACC-037 1:200; Alomone Labs), anti-Cx43 (610062, 1:200; BD Biosciences, Franklin Lakes, NJ, USA), anti-Iba1 (GTX100042, 1:200; GexeTex, Zeeland, MI, USA), anti-iNOS (GTX636531, 1:500; GeneTex), anti-CD206 (A8301, 1:200; ABclonal, Woburn, MA, USA), and anti-ATF3 (sc-518032, 1:200; Santa Cruz). .. Then, the sections were incubated with fluorescent secondary antibodies (Thermo Fisher Scientific) for 1 h at room temperature and mounted using media with 4′,6-diamidino-2-phenylindole (Abcam).

Blocking Assay:

Western Blot:

Article Title: Estradiol activates the CaMKKβ/AMPK pathway to enhance neurite outgrowth in cultured adult sensory neurons.
Article Snippet: Blots were incubated in SignalFire ECL Reagent (Cell Signaling, Danvers, Massachusetts, USA) or Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) and imaged using a ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA). .. Table 1: Primary Antibodies used for Western blotting Primary Antibody Dilution Supplier Anti-pAMPK Thr172 1:1000 Cell Signaling, Danvers, Massachusetts, USA Anti-Total AMPK 1:5000 Abcam, Waltham, Massachusetts, USA Total OxPhos 1:1000 Thermo Fisher Scientific, Waltham, Massachusetts, USA Anti-Total ERK 1:1000 Santa Cruz Biotechnology Inc., Dallas, Texas, USA Anti-PGC-1α 1:1000 Thermo Fisher Scientific, Waltham, Massachusetts, USA Anti-ATF3 1:500 Santa Cruz Biotechnology Inc., Dallas, Texas, USA Anti-ERα 1:1000 Abcam, Waltham, Massachusetts, USA Anti-ERβ 1:1000 Thermo Fisher Scientific, Waltham, Massachusetts, USA Jo ur na l P re -p ro of .. We used the Seahorse XF24 Analyzer (Agilent Technologies Inc, Santa Clara, California, USA) to measure the levels of basal mitochondrial oxygen consumption rate (OCR), the maximal respiration, and the spare respiratory capacity.

Knock-Out:

Article Title: Super enhancer acquisition drives expression of oncogenic PPP1R15B that regulates protein homeostasis in multiple myeloma
Article Snippet: The following antibodies were validated by Cell signaling technology (https://www.cellsignal.com/): anti-ATF6(CST#65880S), anti-CHOP (CST#2895S), anti-cleaved PARP (CST#9541S), anti-cleaved caspase 3 (CST#9661), anti-cleaved caspase 7 (CST#9491), anti-eIF2! (CST#9722S), anti-SP1 (CST#5931), anti-NR2C2 (CST#68191), anti-IRE1 (CST#3294S), anti-PERK (CST#5683S), anti-p70 S6K (CST#2708), anti-Phospho-eIF2 alpha (Ser51) (CST#9721S), anti-Phospho-mTOR (Ser2481) (CST#2974), anti-Phospho-mTOR (Ser2448) (D9C2) (CST#5536), anti-Phospho-PERK (Thr980) (CST#3179S), antiphospho-4E-BP1 (Thr37/46) (CST#2855), anti-phospho-4E-BP1 (Thr70) (CST#9455), anti-phospho-4E-BP1 (Ser65) (CST#9451), anti-4EBP1(CST#9644), anti-YY1 (CST#63227S), anti-p300 (CST#4771S), anti-TSC2 (CST#4308), anti-TSC2 (Ser939) (CST#3615), anti-TSC2 (Thr1462) (CST#3617), anti-TSC2 (Ser1387) (CST#5584), anti-Acetyl-Histone H3 (Lys27) (CST#8173), normal Rabbit IgG (CST#2729S), anti-mouse IgG, HRP-linked (CST#7076) and anti-rabbit IgG, HRP-linked (CST#7074). .. The following antibodies were validated using knockout or overexpression studies by Santa Cruz (https://www.scbt.com/): anti-ATF1 (sc-243), anti-ATF3 (sc-81189), anti-" actin (sc-47778 HRP), anti-caspase 12 (sc-21747), anti-CEBPB (sc-7962, sc-7962X), anti-CEBPG (sc-517003), anti-CREB1 (sc-377154), antiELK4 (sc-1426), anti-GABPA (sc-28312), anti-SP2 (sc-17814) and anti-PIK3C2B (sc-100407). .. The following antibodies were validated by Abcam (https://www.abcam.com/): anti-ATF4 (ab184909), anti-BiP (ab21685), anti-NOXA (ab13654), anti-Phospho-IRE1 (Ser724) (ab48187) and anti-PUMA (ab9643).

Over Expression:

Staining:

Article Title: Endothelial activating transcription factor 3 promotes angiogenesis and vascular repair in the mouse retina
Article Snippet: The following day, the retinas were incubated with the appropriate secondary antibodies for 2 h. Fluorescein images were acquired using an Olympus SV3000 confocal microscope (Olympus, Tokyo, Japan). .. Antibodies for staining were anti-PECAM1 (1/200, BD Biosciences), anti-ERG (1/200, Abcam, Cambridge, UK), anti-ATF3 (1/200, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-ATF3 (1/100, Cell Signaling Technology, Danvers, MA, USA), anti-CD140α (PDGFRα) (1/200, BD Biosciences), anti-Iba1 (1/200, Fujifilm Wako Co., Osaka, Japan), anti-Desmin (1/200, Abcam), anti-PDGFRβ (1/200, Cell Signaling Technology), anti-ESM1 (1/200, R&D systems, Minneapolis, MN, USA), anti-ki67 (1/200, Invitrogen), DAPI (1/1000, Biotium, Hayward, CA, USA), and AlexaFluor® 488-conjugated isolectin GS-IB4 (40 μg/mL, Invitrogen). .. The secondary antibody was Alexa Fluor 488/568/594/647-conjugated IgG (1/500, Invitrogen).

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